Automated C-Terminal Protein Sequence Analysis Using the HP G1009A C-Terminal Protein Sequencing System

Peptide and protein molecules are composed of amino acid molecules covalently coupled end-to-end through peptide bonds, resulting in linear sequences of the amino acid residues. These polypeptide molecules have two chemically distinguishable termini or ends, identified as the amino (N) terminus and the carboxy (C) terminus. The N-terminal and C-terminal amino acid residues define the ends of the amino acid sequence of the polypeptide as depicted in Fig. 1.